South Bay Bio

South Bay Bio is a part of AdipoGen Life Sciences, who also cooperates with a number of other manufacturers such as: Ancell Corporation, BioViotica, Chemodex, Chimerigen Laboratories, Innaxon and JaICA.

Bio-Connect supplies all their products in the Benelux. If you have any questions, please contact the helpdesk.

Major product lines from South Bay Bio

• Proteasome
• UB conjugation
• UB deconjugation
• C-terminal derivatives
• TR-FRET

NEW E3 Ligase TR-FRET Assays

South Bay Bio’s homogeneous Real-Time TR-FRET ubiquitin conjugation assays are simple; the format is 96 or 384 well low-volume plates (making them well suited for HTS). Using ubiquitin either labeled with Europium-Cryptate (donor) or Cyanine 5 (acceptor), for the first time ubiquitin conjugation and deconjugation can be measured homogenously in Real-Time (facilitating enzyme kinetics or endpoint if preferred), with assays commonly exhibiting Z’ 0.8 and a Signal to Noise of commonly >3000%.

Auto-ubiquitination kinetics of several human recombinant E3 ligases of significant interest, namely MDM2, MuRF1, ITCH and Parkin, along with ubiquitin conjugation on substrates such as p53 and s5a have been validated, as well as TR-FRET based deconjugation kinetics of several auto-ubiquitinated ligases using USP2cd and USP7. Coupled with the assays’ short development time (as no antibody development is required for assay optimization), the assay platform is ideally suited for a wide variety of academic and industry screening applications.