Solis BioDyne’s SolisFAST® inhibitor tolerance white paper

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PCR inhibitors prevent the amplification of DNA during the PCR, usually by either interacting with DNA or DNA polymerase, which may affect the sensitivity of the reaction or lead to false negative results [ref. 1]. To avoid this, samples are generally filtered, and DNA is purified [ref. 1]. Unfortunately, removing the inhibitor(s) completely is not always possible. Once this happens, the inhibitors can bind to the DNA, preventing access to it [ref. 1]. They can also reduce the availability of cofactors or interfere with their interaction with DNA polymerase [ref. 1]. Therefore, a good inhibitor-tolerant PCR mix is very useful to avoid these kinds of inhibitor-related problems.

Solis BioDyne’s SolisFAST® DNA Polymerase is an in silico designed enzyme with fast extension rates and a programmed tolerance to inhibitory substances. Combining that with an optimized buffer, the SolisFAST® qPCR Mixes enable robust qPCR performance and accurate target detection under demanding conditions. Together with their Stability TAG technology, the mixes are not just inhibitor tolerant, but also stable at room temperature for up to six months, depending on the product [ref. 2, 3 and 4].

References

1. Schrader C, Schielke A, Ellerbroek L, Johne R. PCR inhibitors – occurrence, properties and removal. J Appl Microbiol. 2012;113(5):1014-1026.
2. Kahre, O., Artma, K., Kahre, T. (2015). Compositions for increasing polypeptide stability and activity, and related methods (European Patent No. 2501716 B1). European Patent Office.
3. Kahre, O., Artma, K., Kahre, T. (2016). Compositions for increasing polypeptide stability and activity, and related methods (US Patent No. 9,321,999 B2). U.S. Patent and Trademark Office.
4. Kahre, O., Artma, K., Kahre, T. (2017). Compositions for increasing polypeptide stability and activity, and related methods (South Korea Patent No. 10-1773636 B1). Korean Intellectual Property Office