By using complementarity-determining region (CDR) grafting and computational molecular modeling, Sino Biological is able to provide monoclonal antibody humanization service with a high humanization rate (>95%). Sino Biological also offers an AI-enabled platform for antibody affinity maturation, which can effectively rank up to 1010 antibody sequences based on their predicted affinity to one or more antigens within a few days. It helps to quickly obtain high-affinity antibodies and speed up the drug development processes.
Service details
Item
Description
Timeline
Deliverable
Antibody humanization
Affinity validation
3-4 weeks
• Top 3 humanized antibodies, 0.5 mg/clone (at least 1 humanized Ab with affinity comparable with parental Ab) • Antibody sequences • Plasmids containing H and L chains • CoA
Mouse antibody/antigen affinity validation
Preparation of human-mouse chimeric antibody
• Transient expression and purification • Affinity validation
Preparation of humanized variants
• 3D structural modeling of antibody • Back mutation
• Transient expression and purification • Affinity validation
Antibody affinity maturation
Mutagenesis Library Generation
4-6 weeks
• Top 3 humanized antibodies, 0.5 mg/clone (at least 1 humanized Ab with affinity comparable with parental Ab) • Antibody sequences • Plasmids containing H and L chains • CoA
AI-Powered Antibody Affinity Prediction
Transient expression and purification
Affinity validation
Case study
Humanization case
The humanized antibody has comparable affinity with the parental antibody
Mouse antibody KD : 6.38E-12M
Humanized antibody KD : 3.90E-12M
Affinity maturation case
Affinity increase from 10-9 to 10-11, up to 218 fold higher
Direct contact technical support
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