Recombinant DNA and RNA Research Antibodies

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At the heart of biology lie the molecules DNA and RNA, which hold the code for all living things. Antibodies against these molecules and associated structures can help elucidate the role of genes and genetic material in processes like gene expression, aging, cancer progression, and infectious disease. To the best research tools, Absolute Antibody recombinantly produces antibodies against targets like Z-DNA, G-quadruplexes, and DNA/RNA hybrids (often an indicator of R-loops) to enable the precise detection and manipulation of important molecules and structures in genomes and cells.

Benefits of Recombinant DNA and RNA Research Antibodies

At Absolute Antibody, our antibody engineers specialize in producing antibodies recombinantly that keep performance and effectiveness in mind. By choosing clinically relevant proteins and antibody clones, swapping antibody species, and tailoring effector function, we maximize the experimental potential of our antibodies. Recombinant antibodies offer several benefits in DNA and RNA research:

• Ensured Reproducibility and Definition: Recombinant antibody production ensures consistent quality across batches, eliminating variability from animal-based production methods and allowing for reliable, repeatable results in DNA/RNA experiments.
• High Purity and Quality: The use of a serum-free, chemically defined expression system results in antibodies with minimal contamination, ensuring high purity and low endotoxin levels, which is critical for the accuracy of DNA/RNA assays.
• Customization and Engineering: Our off-the-shelf antibodies are engineered into various species, isotypes, or formats, including Fc Silent™ formats for abrogated Fc region binding, providing researchers with flexible tools to adapt to various experimental setups. These custom-engineered antibodies open new possibilities for studying DNA/RNA structures, interactions, and functions.

Recombinant Tools
DNA and RNA Research

Anti-dsRNA [J2] Antibody (Ab01299)

This antibody is the recombinant version of the highly cited J2 clone and detects double-stranded RNA (dsRNA), which is generated during viral replications. This tool is useful for virology and has been cited in immunoprecipitation (IP), ELISA, electron microscopy (EM), immunofluorescence (IF), and immunohistochemistry (IHC). Absolute Antibody offers this antibody in a variety of formats, including mouse IgG2a with and without Fc Silencing (Ab01299-2.0 and Ab01299-2.3), mouse Fab fragment (Ab01299-1.6), and more.

Anti-DNA-RNA Hybrid [S9.6] Antibody (Ab01137)

This antibody demonstrates high specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids, making it an effective tool for detecting R-loops, without cross-reacting with single-stranded DNA, double-stranded DNA, RNA, or ribosomal RNA. This antibody has been published in diverse applications, including IF, IP, immunocytochemistry (ICC), proximity ligation assay (PLA), fluorescence in situ hybridization (FISH), and many more. Absolute Antibody offers this antibody in mouse IgG2a (Ab01137-2.0), rabbit IgG (Ab01137-23.0), mouse Fab fragment (Ab01137-24.1), and more.

Anti-DNA/RNA G-quadruplex [BG4] Antibody (Ab00174)

This antibody binds selectively to G-quadruplexes in DNA and RNA. G-quadruplexes are formed by stacked guanine bases that form 4-stranded structures and are implicated in fields of research relevance like cancer development, as they impact genomic stability. This antibody has been published in ELISA, IF, and electrophoretic mobility shift assay (EMSA). Absolute Antibody provides this antibody in a variety of engineered formats, including mouse IgG1 (Ab00174-1.1), human and mouse Fab fragments (Ab00174-10.6 and Ab00174-1.6), and more.

Conclusion

Antibodies targeting DNA, RNA, and associated structures can open a world of understanding. Recombinant engineering further expands the research possibilities by providing species-swapped formats, tailored effector function, and complete biological definition for more specific and reliable antibody performance.

Absolute Antibody antibodies are available off-the-shelf in diverse formats, isotypes, subtypes, and species. If they don’t have just what you need, they offer custom services such as antibody engineering. Contact your account manager for more information.