11 February 2025

PromoCell: World-class Cells and Media for Primary Cell Culture

Cryo-SFM Plus is a defined, protein- and animal component-free cryopreservation medium, with or without phenol red. It ensures exceptional cell integrity and adhesion after thawing for sensitive and diverse applications.


PromoCell GmbH is a leading biotech company dedicated to advancing scientific discovery. With over 30 years of expertise, they are globally recognized for providing scientists with essential tools and support for groundbreaking research.

PromoCell offers a world-class portfolio of human cell culture products:

  • primary cells
  • stem cells
  • blood cells
  • media, cancer media, GMP grade media
  • buffers & solutions

In this article, we highlight PromoCell’s Cryo-SFM Plus, a solution designed for the optimal preservation of cells.

PromoCell’s Cryo-SFM Plus: For perfectly preserved cells

Cryo-SFM Plus sets a new standard in cryopreservation with its innovative, defined and protein- and animal component-free formulation. Specifically designed to optimize cell integrity and functionality after freezing and thawing, this medium is an excellent choice for preserving human primary cells, stem cells, and other sensitive cell types such as human induced pluripotent stem cells (hiPSCs).

Download: Specification of PromoCell’s Media & Reagents

Key Benefits of Cryo-SFM Plus

  • Animal component-free and protein-free formulation minimizing the risk of contamination and variability in cell cultures.
  • High cell viability, integrity and adhesion post-thaw.
  • Defined and consistent composition for reproducible results.

PromoCell now offers Cryo-SFM Plus in two variants

  • With phenol red: Ideal for general applications as phenol red serves as a pH indicator during the cryopreservation process.
  • Phenol red-free: Perfect for customers who may require a phenol red-free system to avoid potential interference during freezing, subsequent cultivation, or sensitive downstream applications such as fluorescence-based assays, live-cell imaging, and high-throughput screening. Additionally, it is ideal for those who wish to avoid phenol red due to concerns about estrogen sensitivity in their cells.

The flexibility offered by the two variants allows researchers and industrial users to select the formulation that best suits their specific needs. Whether you are working in drug discovery, cell-based assays, or bioproduction, Cryo-SFM Plus delivers reliability and performance for your cryopreservation workflows.

Cryo-SFM Plus is developed using an antioxidant technology, minimizing oxidative stress during freezing and thawing. The formulation ensures superior cell recovery and functionality by minimizing cell damage caused by ice crystal formation during the freezing process. This innovative approach is protected under a registered patent*, guaranteeing customers an exclusive, state-of-the-art solution for cryopreservation.

This leads to superior recovery rates and excellent adhesion properties after thawing. The medium is manufactured under stringent quality standards to ensure batch-to-batch consistency, reproducibility, and compliance with regulatory guidelines due to its defined and animal component-free formulation.

Fig.1: Comparison of post-thaw growth of hiPSCs

Comparison of post-thaw growth of human induced pluripotent stem cells (hiPSCs) cryopreserved in different freezing media. Cell frozen using Cryo-SFM Plus exhibit superior survival, attachment, and proliferation after thawing.

We continue to innovate with its Human Centered Science philosophy, ensuring that researchers and industrial users have access to cutting-edge cell culture solutions. Choose Cryo-SFM Plus—available with or without phenol red—to enhance the efficiency and reliability of your cryopreservation processes.

Optimize your workflow today
with Cryo-SFM Plus from PromoCell

Download the cell cryopreservation in Cryo-SFM Plus Application note



* PromoCell’s technology is patented in Germany and pending approval in other countries.

PromoCell: World-class Cells and Media for Primary Cell Culture

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