High-throughput Antibody Production in Just 10 Days

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Service Highlights

Short lead time: From gene to purified antibody as fast as 10 days

Dual production centers: Beijing & Taizhou, larger production capacity and more convenient

Multi-analysis: SDS-PAGE, SEC-HPLC, endotoxin, WB, ELISA, SPR/BLI, FACS, etc.

High throughput: >1000 Ab /project

Advanced equipment: Equipped with automatic molecular construction platform, more accurate and efficient

Superior quality>95% purity by SEC-HPLC, <1 EU/mg endotoxin level

Service Details for High-throughput Antibody Expression

Timeline: 10-21 days
Production scales: 50μg – 5mg

• Gene synthesis and codon optimization (optional)

Starting from antibody sequences  

• Vector construction 

Cloning into expression vectors
Plasmid sequencing
Plasmid production 

• High-throughput expression and purification

Transient transfection of HEK293/CHO cells
Antibody purification 

• QC analysis 

SDS-PAGEUVSEC-HPLC (optional)
Endotoxin testing (optional) 

• Deliverables 

Purified antibodies
CoA
Expression vector (optional)
Cell culture supernatant (optional)

Case Study of HTP Gene to Antibody Production

Sino Biological can achieve high-throughput gene-to-antibody manufacturing in mammalian cells. A client requested the expression of recombinant full-length antibodies on a small scale. The preparation cycle was only 10 days, and the monomer purity reached 99%.

SDS-PAGE

SEC-HPLC >99%

Our Equipment

AKTA

Biacore

HPLC

Oclet

GeniRobot

Incubator Shakers