DNAbsolute – A column-free DNA extraction buffer

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How does it work ?

DNAbsolute is composed of an ionic liquid that has a selective and high affinity for DNA strands. When mixed with your sample, the DNAbsolute reagent will directly bind DNA and precipitate it without the need for RNase treatment, protein precipitation or use of hazardous reagents.

What types of samples can it be applied to?

Since its release, DNAbsolute has been successfully used to extract DNA from a wide panel of samples, including some of the most challenging ones:  

• Insects (drosophila, psyllids, ants, beetles, butterflies, cochineals)
• Fish (shark fins)
• Mammals (rodent skin)
• Plants (dried leaves)
• Corals
• Bacteria (Salmonella, XL-1 blue)
• Mussels
• Cnidarians

Key advantages

• Non-toxic: No need to work under a chemical hood since DNAbsolute doesn’t include any harmful chemical compounds.
• Fast & Simple: Based on ionic liquids, the DNAbsolute method does not involve any plastic columns. Therefore, there is no restriction of volume. DNAbsolute is the go-to solution for researchers seeking a rapid, user-friendly, and eco-conscious DNA extraction solution.
• Versatility: DNAbsolute has been successfully used for extracting DNA from insects (drosophila, psyllids, beetles, ants, cochineals), fish (shark skin), mammals (rhodent skin), plants (dried leaves), corals (dried and ethanol-preserved), bacteria (Salmonella), mussels and cnidarians.
• Sensitivity: Allowing efficient DNA recovery from single specimens down to a single leg (insects), DNAbsolute is a solution of choice to get the most of museum specimens, limited or precious samples. 
• Cost-effective: DNAbsolute was shown to be effective on a single beetle leg, which require a very low volume of DNAbsolute. Depending on the initial sample, the cost per extraction can go down to 0.30€.

How to use DNAbsolute ?

Once your sample is lysed, the whole DNA extraction process should take no more than 30 minutes.

What is included in the DNAbsolute kit? Is there anything I need that is not provided? 

Our DNAbsolute kit contains the DNAbsolute solution as well as some lysis, PBS and Tris buffers. All you need to have on your side is a refrigerated centrifuge, ethanol and proteinase K. You can also add an RNase treatment if needed.