The Cell-free platform developed by Sino Biological allows rapid protein production in vitro without the need for living cells. The expression process has been accelerated from days to only a few hours.
Typical protein synthesis in CFPS systems takes only hours compared to the days to weeks required for cell-based production. Moreover, CFPS systems from Sino Biological enable the production of difficult-to-express proteins and their open nature facilitates direct manipulation of the chemical environment and high-throughput potential.
The CFPS platform has already proven itself with the successful production of proteins and antibodies that are difficult to express in other systems. Sino Biological will be able to provide you with high-quality proteins and antibodies with shorter lead times, accelerating your research and development process.
Highlights
Fast and efficient : synthesis completed in just 3 hours
High success rate : >99%
Consistency: comparable activity to proteins expressed in mammalian cells
Challenging antibody synthesis: successful synthesis of antibody fragments with cellular expression difficulties
Process
Gene synthesis -> Vector Contruction -> Cell-free Protein Synthesis ->
Purification & QC -> Delivery
Cell-free Protein Synthesis -> Purification = as fast as 1 day
Case Studies
High purity VHH synthesis
VHH synthesized by CFPS with purity by SDS-PAGE and SEC-HPLC >95%.
High-troughput scFv synthesis
From gene to purified 50 scFvs, Sino Biological complete synthesis in only 5 days.
VHH synthesized by CFPS with comparable binding activity as that expressed in mammalian cells | anti-IL6R VHH
anti-IL6R VHH
Cell-free
HEK293
EC50 (μg/mL)
0.026
0.034
Measured by its binding ability in a functional ELISA. Immobilized IL6R at 0.5 μg/ml (100 μL/well) can bind anti-IL6R nanobody expressed by CFPS system and HEK293 cell respectively.
anti-TROP2 VHH
anti-TROP2 VHH
Cell-free
HEK293
EC50 (μg/mL)
0.179
0.164
Measured by its binding ability in a functional ELISA. Immobilized TROP2 at 0.5 μg/ml (100 μL/well) can bind anti-TROP2 nanobody expressed by CFPS system and HEK293 Cell respectively.
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Recombinant antibody expression in hours
Purified antibody in 1 day
Fast and efficient : synthesis completed in just 3 hours
High success rate : >99%
Consistency: comparable activity to proteins expressed in mammalian cells
Challenging antibody synthesis: successful synthesis of antibody fragments with cellular expression difficulties
Gene synthesis -> 
Vector Contruction -> 
Cell-free Protein Synthesis -> 
Purification & QC -> 
Delivery
