Antibodies.com launches high-sensitivity 90-Minute ELISAs

Taking just half the time and with a catalog of over 4,300 target antigens, the new 90-Minute ELISA kits from Antibodies.com produce the same accurate and reliable results as traditional ELISAs.

The enzyme-linked immunosorbent assay (ELISA) has become the standard method for in vitro quantification of target antigens in biological fluids, thanks to the high specificity of the antigen-antibody interaction. This is particularly relevant in sandwich ELISAs, in which matched pairs of antibodies bind an antigen (the sandwich “filling”) at two distinct sites, increasing specificity.

90-Minute ELISAs use this same sandwich format to maintain high specificity, but they streamline the protocol to allow researchers to get their results faster. The addition of over 4,300 90-Minute ELISA kits to their portfolio makes Antibodies.com the leading provider of rapid ELISA kits, allowing researchers to target over 3x more targets than other suppliers.

Sandwich ELISAs are one of the most common forms of ELISA, and work by using an immobilized capture antibody to selectively bind to an antigen that is present in a biological sample. The now-immobilized antigen is then recognized by the second half of the matched antibody pair, the detection antibody, which is conjugated to an enzyme such as horseradish peroxidase to produce a colored product on addition of an appropriate substrate. As shown in the figure below, 90-Minute ELISAs use the same general principle but reduce the number of incubation and wash steps compared to a traditional ELISA.

One of the primary concerns in any assay is sensitivity. Researchers often face a trade-off between achieving optimal sensitivity, the time required for the assay, and cost. The 90-minute ELISA delivers optimal sensitivity at an affordable price through the use of the biotin-streptavidin system, without compromising on speed. This allows researchers to spend less time waiting and more time doing what matters, such as interpreting results and planning the next stage of the project. As an additional benefit, 90-Minute ELISAs require a lower sample volume than is typically needed for a conventional ELISA, saving on precious sample and allowing researchers to probe for more targets.

Already well-known for reliable ELISA kits, the launch of 90-Minute ELISAs expands the total ELISA kit catalog of Antibodies.com to over 22,000, targeting a wide range of antigens including cytokines, growth factors and hormones