Air-Liquid Interface Culture System

The standardized Air-Liquid Interface (ALI) Medium from PromoCell in combination with ALI pre-screened Human Bronchial Epithelial Cells enables creation of a reproducible human 3D airway model with stable and predictable epithelial barrier function.

PromoCell now has a serum- and bovine pituitary extract (BPE)-free ALI Medium (ALI-Airway) which closely models the airway epithelium and exhibits morphological and functional characteristics similar to those of the human airway in vivo.

Well-differentiated in vitro airway epithelial cultures allow study of the physiological and pathophysiological responses of the respiratory tract in basic respiratory research, toxicity studies, infectious disease research, and drug development.

Together with their ALI pre-screened Human Bronchial Epithelial Cells (HBEpC) they provide a complete Air-Liquid Interface Culture System to facilitate reproducible and physiologically relevant respiratory research. Their ALI pre-screened HBEpCs cultured in ALI-Airway medium form tight junctions with transepithelial electrical resistance (TEER) values of over 500 Ω*cm2. This allows an intact barrier function over a 28 days period.

Advantages of the Air-Liquid Interface Culture System

• Physiologically relevant 3D cell culture system
• Maximized experimental reproducibility with a standardized medium formulation
• Stable and uniform TEER values
• ALI pre-screened HBEpC available on request, optionally also from HLA-typed donors
• Cell viability >70% for differentiated HBEpC

The ALI-Airway is available as a ready-to-use medium, consisting of a 500 ml bottle of Basal Medium and a single vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in complete Growth Medium that’s ready to use.

Read more on PromoCell’s blog on Air-liquid interface culture: getting a closer look into our airways