Unique ELISA for evaluating oxidative stress

Elevated plasma levels of oxidized LDL are associated with higher cardiovascular risk and higher incidence of metabolic diseases, i.e., metabolic syndrome, obesity, and type 2 diabetes. Also, sub-clinical atherosclerosis, kidney, and liver diseases are associated with increased levels of oxidized LDL.

First-to-market test for evaluating oxidative stress

Mercodia’s Oxidized LDL ELISA was launched 20 years ago as the first-to-market test for evaluating oxidative stress. The assay is intended to be used for the in vitro quantitative measurement of oxidized LDL in human serum or plasma and is the most widely used ELISA in oxidized LDL research.

Mercodia’s Oxidized LDL ELISA is used as an aid to investigate risk factors for cardiovascular disease and the addition of oxidized LDL to the established risk factors may improve cardiovascular risk prediction.

Advantages of Mercodia’s Oxidized LDL ELISA

• The most widely used ELISA in oxidized LDL research
• Documented in well over 1,000 peer-reviewed publications
• Used by several large-scale clinical diagnostic/reference labs
• Measures the highly oxidized forms of lipid and aldehyde modified oxidized LDL
• Accurate measurement of the physiological signaling to the scavenging macrophages that will create foam cells, vascular inflammation, and atherosclerosis
• CE/IVD labeled on selected markets
• Controls included in the kit

Unique specificity

The term oxidized LDL is often used to describe a variety of LDL molecules because the composition of this molecule is very heterogeneous depending on the grade of oxidation.

The specificity of the 4E6 mouse monoclonal antibody used in the Mercodia Oxidized LDL ELISA ensures that the assay measures the highly oxidized forms of lipid and aldehyde modified oxidized LDL which are the cause of foam cell formation. This contrasts with other ELISA assays with specificity towards malondialdehyde (MDA) modified LDL, which not only recognizes oxidized LDL with an affinity for scavenger-receptors but also LDL with a lower grade of oxidation.

The unique affinity of the proprietary mouse monoclonal antibody 4E6 used in the Mercodia Oxidized LDL ELISA makes it possible to measure both lipid and aldehyde modified oxidized LDL, which sets this assay apart from assays based on other antibodies.

New research

Discover more in this selection of recently published studies relating to oxidative stress and oxidized LDL:

• Lundberg AK, Chung RWS, Zeijlon L, Fernström G, Jonasson L. Oxidative stress response in regulatory and conventional T cells: a comparison between patients with chronic coronary syndrome and healthy subjects. J Transl Med. 2021;19(1):241.
• Roumeliotis S, Georgianos PI, Roumeliotis A, et al. Oxidized ldl modifies the association between proteinuria and deterioration of kidney function in proteinuric diabetic kidney disease. Life (Basel). 2021;11(6):504.
• Suleiman N, Alkasem M, Hassoun S, et al. Insulin sensitivity variations in apparently healthy Arab male subjects: correlation with insulin and C peptide. BMJ Open Diabetes Res Care. 2021;9(2):e002039.
• Salehi SS, Mirmiranpour H, Rabizadeh S, et al. Improvement in redox homeostaasis after cytoreductive surgery in colorectal adenocarcinoma. Oxid Med Cell Longev. 2021;2021:8864905.
• Schadler P, Lohberger B, Thauerer B, et al. The association of blood biomarkers and body mass index in knee osteoarthritis: a cross-sectional study. Cartilage. 2022;13(1):19476035211069252.

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