Enhance your research with Aurora Biolabs‘ latest collection of wild-type and mutant KRAS recombinant proteins, along with specialized assay kits and services designed for drug discovery and biochemical analysis.
Aurora Biolabs provides a comprehensive selection of KRAS proteins and assays to support your research needs:
Their TR-FRET-based Nucleotide Exchange Assay enables precise measurement of GTP binding to KRAS in the presence of SOS1, a key guanine nucleotide exchange factor (GEF). The assay utilizes a Terbium-labeled anti-Tag2 antibody (HTRF donor) to detect fluorescence-labeled GTP binding to KRAS. Upon excitation at 340 nm, energy transfer occurs, producing a measurable fluorescence signal at 665 nm. This assay provides a quantitative method to evaluate nucleotide exchange activity, with inhibitors reducing the HTRF signal.
Image: KRAS Nucleotide exchange assay schematic
The TR-FRET-based KRAS-cRAF Binding Assay is designed to detect interactions between activated KRAS (loaded with GppNHp) and cRAF. The assay employs a Terbium-labeled anti-Tag2 antibody (HTRF donor) for KRAS and a fluorescence-labeled anti-Tag1 antibody (HTRF acceptor) for cRAF. When KRAS binds to cRAF, fluorescence resonance energy transfer (FRET) occurs, enabling quantitative measurement of the interaction.
Image: Kras-cRAF binding assay schematic
Aurora Biolabs’ KRAS proteins and assays provide reliable tools for studying KRAS signaling pathways, inhibitor screening, and drug development. Contact us to learn more about how these products can support your research.
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